human dpp4 cd26 duoset elisa  (R&D Systems)

Journal: Immunity

Article Title: Immuno-proteomic profiling reveals aberrant immune cell regulation in the airways of individuals with ongoing post-COVID-19 respiratory disease

doi: 10.1016/j.immuni.2022.01.017

Figure Lengend Snippet: CXCR3 ligands and markers of epithelial damage correlate with CD8 T cells numbers in the airways BAL immune cells and protein concentrations were analyzed post-COVID-19 infection. (A) Heatmap displaying the relationship between proteins and immune cell frequencies. The proteins and immune cell traits displayed are those with at least one significant (5% FDR) association from linear regression analyses (see K). (B and C) For each sample, protein concentrations for CXCL9, CXCL 10, and CXCL 11 were normalized to the median concentration in healthy controls. For each sample, the mean of the normalized values for the 3 proteins was calculated to provide a summary metric for CXCR3 chemokines. This was then plotted against versus (B) T, NK, and NKT proportions in post-COVID-19 patients and healthy controls and (C) monocyte frequencies and subsets in post-COVID-19 patients only. (D) BAL T cell frequency versus CD4 and CD8a concentrations as measured by Olink. (E) CD8a concentration versus CASP3, EPCAM, MB, and DPP4 in the airways. (F) CXCL9, CXCL10, and CXCL11 concentration in in the BAL were measure by legendplex. (G) DPP4, albumin, and LDH concentrations in the BAL determined by ELISA. Data are presented as median ± IQR. (A) Pearsons correlation of n = 19 post-COVID-19 patients, the r value is shown. (B–E) Each point represents an individual patient, linear regression line ±95% confidence intervals are depicted, and r and p values from Pearsons correlation are stated. (F and G) Represents n = 38 post-COVID-19 and n = 20 healthy control individuals. Statistics were conducted using Mann-Whitney U test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.005, ∗∗∗∗ p < 0.001. pCOVID = post-COVID-19.

Article Snippet: DPP4 (R&D systems, DY1180) and albumin (Bethyl Laboratories, E80-129) concentrations in the BAL were quantified by ELISA according to manufacturer’s instructions.

Techniques: Infection, Concentration Assay, Enzyme-linked Immunosorbent Assay, Control, MANN-WHITNEY

Journal: Immunity

Article Title: Immuno-proteomic profiling reveals aberrant immune cell regulation in the airways of individuals with ongoing post-COVID-19 respiratory disease

doi: 10.1016/j.immuni.2022.01.017

Figure Lengend Snippet: Distinct airway proteomic and immune cell phenotypes correlate with distinct indicators of respiratory pathology post-COVID (A) Immune cell proportions in the BAL, as a percentage of total leukocytes, BAL albumin (μg/mL), LDH (OD450), and DPP4 (ng/mL) were correlated with CT (% abnormality) or FEV1, FVC, and TLCO (% of predicted normal). Spearman’s rho is displayed as a heatmap. (B) Albumin (μg/mL), LDH (OD450), and DPP4 (ng/mL) in the BAL segregated by CT abnormality (%), predicted FVC (%), and predicted TLCO (%). (C) The number of major immune cell population per mL of BAL versus CT abnormality, FVC, and TLCO. (D) Total number of monocyte subsets per mL BAL was segregated by CT, FVC, and TCLO. (E) BAL CXCL8 (pg/mL) measured by Legendplex in HC and post-COVID-19 patients and correlated versus total neutrophil numbers (per mL/BAL). (F) BAL CXCL8 (pg/mL) measured by legendplex in post COVID-19 patients segregated by CT abnormality (%), predicted FVC (%), and predicted TLCO (%). (G) BAL CCL2 (pg/mL) measured by legendplex in HC and post COVID-19 patients and correlated versus myeloid cells (CD11b + ) in the BAL. (H) BAL CCL2 (pg/mL) measured by legendplex in post COVID-19 patients segregated by CT abnormality (%), predicted FVC (%), and predicted TLCO (%). Where applicable individual points are shown, and data are presented as median ± IQR. Each point represents an individual patient. Statistical significance for (B–H) was tested by Mann-Whitney U test. Benjamini-Hochberg adjusted (5% FDR) p values ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.005, ∗∗∗∗ p < 0.001. Pearson’s correlations were performed in (E and G), r and p values are shown, as is a line of best fit ±95% confidence intervals. See and .

Article Snippet: DPP4 (R&D systems, DY1180) and albumin (Bethyl Laboratories, E80-129) concentrations in the BAL were quantified by ELISA according to manufacturer’s instructions.

Techniques: MANN-WHITNEY

Journal: Immunity

Article Title: Immuno-proteomic profiling reveals aberrant immune cell regulation in the airways of individuals with ongoing post-COVID-19 respiratory disease

doi: 10.1016/j.immuni.2022.01.017

Figure Lengend Snippet: Increased airway T cell and B cell abundance is associated with more severe ongoing respiratory pathophysiology post-COVID-19 (A) Immune cell proportions in the BAL, as a percentage of total leukocytes, BAL albumin (μg/mL), LDH (OD450), and DPP4 (ng/mL) were correlated with BAL albumin, LDH, and DPP4 concentrations. (B and C) (B) BAL T cell subtypes and (C) subsets of CD4 and CD8 T cells were analyzed against FVC. (D) B cells subsets numbers per mL BAL segregated by CT, FVC, and TLCO. (E) Total and RBD-specific IgA and IgG were measured in the BAL and plasma. (F) Antibody concentrations were correlated with BAL and plasma B cell subsets of total leukocytes. (G) Antibody concentrations measured in BAL and plasma segregated by CT, FVC, and TLCO. (H) Antibody concentrations were correlated with BAL CD4 and CD8 T cells and their subsets as a proportion of total leukocytes. (A, F, and H) Spearman correlation. Correlations p < 0.05 after Benjamini-Hochberg adjustment for an FDR of 5% are indicated by thickened boxes. (B–E and G) was tested by Mann-Whitney U test. Benjamini-Hochberg adjusted (5% FDR) ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.005, ∗∗∗∗ p < 0.001. (A, F, and G) are display Spearman’s rho correlation.

Article Snippet: DPP4 (R&D systems, DY1180) and albumin (Bethyl Laboratories, E80-129) concentrations in the BAL were quantified by ELISA according to manufacturer’s instructions.

Techniques: Clinical Proteomics, MANN-WHITNEY

Journal: Immunity

Article Title: Immuno-proteomic profiling reveals aberrant immune cell regulation in the airways of individuals with ongoing post-COVID-19 respiratory disease

doi: 10.1016/j.immuni.2022.01.017

Figure Lengend Snippet: Reduced cellularity is observed in the airways 1 year after initial bronchoscopy post-COVID-19 (A) % lung CT abnormality or predicted FVC (%) or TLCO (%) at first appointment and 1 year follow up (n = 17 pCOVID-19 patients). (B) Total cell counts and cell counts of lymphocyte populations, macrophages, neutrophils, and monocyte subsets in the BAL. (C and D) (C) Proportions of T cell subsets and (D) CD4 and CD8 CD69 + CD103 + as a proportion of BAL T cells. (E) Proportions of memory (CD27 + IgD − ) and plasmablasts (CD27 + CD38 + ) of CD20 + B cells in the BAL. (F) DPP4, LDH, and albumin measurements in BAL. All data depict first bronchoscopy between 3–6 months post discharge and at 1 year post discharge. Each point represents a single patient. (B–E) Represent n = 3 patients. Green shading indicates median ± IQR for proportions of populations and mediator concentration observed in healthy airways. (A) Wilcoxon matched-pair signed rank test. ∗ p < 0.05, ∗∗∗ p < 0.001.

Article Snippet: DPP4 (R&D systems, DY1180) and albumin (Bethyl Laboratories, E80-129) concentrations in the BAL were quantified by ELISA according to manufacturer’s instructions.

Techniques: Concentration Assay

Journal: Immunity

Article Title: Immuno-proteomic profiling reveals aberrant immune cell regulation in the airways of individuals with ongoing post-COVID-19 respiratory disease

doi: 10.1016/j.immuni.2022.01.017

Figure Lengend Snippet:

Article Snippet: DPP4 (R&D systems, DY1180) and albumin (Bethyl Laboratories, E80-129) concentrations in the BAL were quantified by ELISA according to manufacturer’s instructions.

Techniques: Recombinant, Staining, Ii Assay, Enzyme-linked Immunosorbent Assay, Lactate Dehydrogenase Assay, Software